Kiranjeet Kaur (Biotechnologist)

  Earthly Sky Glow (Light Pollution: Introduction, Types, Causes, Effects, and Prevention) Photo/Unsplash   “Environmental pollution is an incurable disease. It can only be prevented”– Barry Commoner.   Pollution involves introducing harmful materials, known as pollutants, into the environment. This pollution can cause damage to the quality of our environment, leading to degradation and environmental crises. Pollutants can result from human activities or natural disasters. In this article, we will discuss light pollution. Light is electromagnetic radiation that can be perceived by the human eye to see an object. Light shows interaction with matters that helped shape the structure of the universe. Excess of everything is bad because, as we know, "too much greed is not good." The excessive use of artificial light sources during the day and night causes pollution. It acts as a pollutant, causing light pollution that harms our environment." Light Pollution T

  List of 17 Enzymes with their function that are used in recombinant DNA technology /Genetic Engineering     1.      Alkaline phosphatase : This enzyme helps to remove the phosphate groups from 5’-ends of double and single standard DNA and RNA. It also helps prevent re-circularization during cloning and dephosphorylate DNA before Kinase labeling protocols. 2.   Terminal Transferase : Enzyme very useful in homopolymer tailing. It helps to add nucleotides to the 3’ends of DNA or RNA. This enzyme is used as a template for a primer in subsequent PCR. 3.      Bal 31 nuclease : In DNA technology this enzyme is used to degrade both 3’ and 5’ termini of duplex DNA without generating any internal scissions. In short, this enzyme is used for the progressive shortening of DNA. 4.     S1nuclease : It is an endonuclease enzyme that helps to degrade single-standard DNA and RNA but can’t degrade double-standard DNA or RNA-DNA hybrids. 5.      DNA Ligase : In DNA replication, the DNA